Predicting the development and progression of primary open-angle glaucoma based on the determination of GST gene polymorphisms
Journal Title: Офтальмологический журнал - Year 2018, Vol 0, Issue 4
Abstract
Background: Epidemiological studies have demonstrated that primary open-angle glaucoma (POAG) is a complex multifactorial disease resulting from interaction of risk factors and gene polymorphisms. Previously, we have demonstrated the role of deletion polymorphisms of glutathione-S-transferase (GST) genes in the development and progression of POAG. Purpose: To predict the speed of POAG development and the speed of POAG progression as early as at patient presentation based on determination of GST gene (GSTТ1, GSTР1 and GSTM1) polymorphisms. Materials and Methods: Data of 172 patients (344 eyes) diagnosed with stage 1, 2, 3 or 4 POAG, and of 98 control group volunteers (196 eyes) diagnosed with no POAG, were included in the analysis. Two indices, SDPOAG (speed of POAG development, expressed as stage/life year), and SPPOAG (speed of POAG progression, expressed as stage/disease year), were used as measures for assessing speed of the pathological process. Polymorphisms of GSTP1, GSTM1 та GSTT1 were investigated by real-time polymerase chain reaction (PCR) using TaqMan Mutation Detection Assays (Life Technologies, Carlsbad, CA) and Gene Amp® 7500 PCR System (Applied Biosystems, Foster City, CA). Results and Discussion: We built an algorithm (computer program) for predicting the speed of the development and progression of POAG, with the potential to calculate these indices as early as at presentation. The GSTP1 Ile105Val polymorphism (with the presence of the mutant Val allele) and GSTM1 and GSTT1 deletion polymorphisms contributed more to prediction of dependent variables, SDPOAG and SPPOAG, than others. We found a significant effect of the combination of independent variables in the regression equation on the dependent variable SDPOAG, with a multiple correlation coefficient R of 0.801, a coefficient of determination R2 of 0.622, and an F value of 77.338 at p<0.0001. In addition, the regression equation for prediction for the speed of the progression of POAG had a multiple correlation coefficient R of 0.857, a coefficient of determination R2 of 0.734, and an F value of 73.450 at p<0.0001. Conclusion: The objectives of the study (i.e., to predict the age of onset of clinical manifestations of POAG and the speed of the development of POAG, with the potential to calculate them as early as at presentation) have been met.
Authors and Affiliations
S. Rykov, A. Burdei
Keywords
Related Articles
- EP ID EP423675
- DOI 10.31288/oftalmolzh201841116
- Views 122
- Downloads 0
Influence of the fetal neuropeptide complex on changes in retinal light sensitivity over time in patients with primary open-angle glaucoma
Purpose: To investigate the influence of a fetal neuropeptide complex on changes in retinal light sensitivity over time in patients with primary open-angle glaucoma (POAG). Materials and Methods: The study involved 104...
Targeted transscleral laser photocoagulation of the ciliary body in patients with neovascular glaucoma
Transscleral laser photocoagulation of the ciliary body is used to reduce intraocular pressure (IOP) in neovascular glaucoma, which is associated with a risk of complications. Purpose. To study the efficacy and safet...
Indications for inpatient treatment of cataract
Purpose: To develop indications for inpatient cataract treatment based on the retrospective review of case histories of cataract patients. Materials and Methods: We retrospectively reviewed 636 case histories of catarac...
Changes in the retinal thickness after microinvasive vitrectomy with inner limiting membrane removal in patients with diabetic retinopathy
The purpose. To study changes in the macular retinal thickness in patients with diabetic retinopathy after microinvasive vitrectomy with inner limiting membrane (ILM) removal. Material and Methods. This was a retrospect...
Blood sICAM-1 levels in type 2 diabetes mellitus patients with various grades of DME
Background: Diabetic macular edema (DME) is a major cause of visual impairment in type 2 diabetes mellitus (DM) patients. Non-specific inflammation is an important factor of the underlying processes of DME. Intercellular...