Reaction between cationic detergent and Coomassie Brilliant Blue G-250: application to urinalysis
Journal Title: Journal of Analytical Bio-Science - Year 2013, Vol 36, Issue 4
Abstract
In the qualitative test for urine protein by a test strip, a false-positive reaction due to a cationic detergent is well known. A method which can discriminate a false-positive reaction was studied, using CBB G-250 reagent and benzalkonium chloride. At pH 11, benzalkonium chloride gave a pink color with an absorption maximum at 540 nm, but HSA did not produce any colored product by the CBB G-250 reagent. It is found that the difference in this color development by the CBB G-250 reagent can be used to discriminate a false-positive reaction due to cationic detergent.
Authors and Affiliations
Yuji Suzuki
Keywords
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