SEARCHING FOR LEPTIN GENOTYPE, PROLACTIN RECEPTOR GENOTYPE AND CHOSEN ENVIRONMENTAL FACTORS INFLUENCE ON SPERM QUALITY IN BOARS ([i]SUS SCROFA DOMESTICA[/i])
Journal Title: Zeszyty Naukowe Uniwersytetu Przyrodniczego we Wrocławiu, Biologia i Hodowla Zwierząt - Year 2011, Vol 583, Issue 0
Abstract
The aim of the study was the statistical analysis of the influence of leptin genotypes ([i]LEP[/i]), prolactin receptor genotypes ([i]PRLR[/i]) and selected environmental factors on boar’s semen traits. 89 boars with known leptin genotype and 89 with known prolactin receptor genotype were examined. They belonged to 10 breed groups kept in AI station in 1996–2002. 9285 ejaculates were collected from boars with known genotype, and 9708 ejaculates from those with PRLR genotype. Polymorphism in [i]LEP [/i]and [i]PRLR [/i]was detected with PCR-RFLP method with AluI restrictive enzyme for [i]PRLR [/i]gene and [i]HinfI [/i]for [i]LEP [/i]gene. Two alleles were identyfied for [i]LEP [/i]gene: [i]C [/i]and [i]T[/i] with 0,17 and 0,83 frequency, respectively. The genotypes frequency were as follows: [i]CC [/i]– 0,03 [i]CT [/i]– 0,28 and [i]TT[/i] - 0,69. In [i]PRLR [/i]gene [i]A[/i] and [i]B[/i] alleles were identyfied with 0,72 and 0,28 frequency, respectively, whereas the genotypes appeared with frequency: [i]AA [/i]– 0,52, [i]AB [/i]– 0,40, [i]BB [/i]- 0,08. Statistical analysis revealed that different variants of leptin and prolactin receptor genotypes can be significant for studied semen parameters (P≤0,01). Boars with [i]AB [/i]and [i]BB [/i]prolactin receptor genotype dominated over those with [i]AA [/i]genotype as far as all semen traits were concerned. Another analysis, performed on ejaculates collected from boars with leptin genotype, revealed domination of [i]TT [/i]genotype over other ones as far as semen volume, percentage of alive sperms, number of sperms in ejaculate, total number of sperms in insemination dose and number of insemination doses were concerned. Boars with [i]CC[/i] genotype had the highest sperm concentration in ejaculate. Furthermore, it was demonstrated that effect of insemination station, breed and interaction of these two effects had influence on none of the semen traits. Other effects were statistically significant for semen quality.
Authors and Affiliations
Katarzyna Sieńko, Heliodor Wierzbicki
Keywords
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