Speciation and Antimicrobial Susceptibility Pattern of Clinically Significant Coagulase Negative Staphylococci in a Tertiary Health Care Centre
Journal Title: Scholars Journal of Applied Medical Sciences - Year 2017, Vol 5, Issue 8
Abstract
Abstract:Coagulase negative staphylococci (CoNS) were once considered as skin commensals and dismissed as culture contaminants, are now being recognized as significant nosocomial pathogens. CoNS are also associatedwith resistance to several commonly used antimicrobial agents. It is important to identify CoNS up to the species level, as the epidemiology, the pathogenecity and drug resistance varies from species to species. Study was carried for period of 12 months. CoNS isolates from various clinical samples, collected from different sites were subjected to biochemical characterization. Antimicrobial susceptibility test were done by using Kirby Bauer disc diffusion method.Out of eighty six isolates Staphylococcus epidermidis 29(33.7%) was most common isolate, followed by S.hemolyticus 23(26.7%), S.saprophyticus 11(12.8%), and S.hominis 9 (10.5%). Other CoNS isolates were S.lugdunensis 6(7.0%) S. schleiferi 4(4.7%), S.warneri 2(2.4%), S.cohnii 1(1.2%). Antibiotic susceptibility testing showed maximum resistance to Penicillin and Amoxy-clav with 80%-90% and sensitivity to Gentamicin, Linezolid and Vancomycin (80%-100%). Methicillin resistance was detected in 27(37%) of isolatesDue to emergence of multiple drug resistance among CoNS isolates, there is a need to adopt simple laboratory procedures to identify, speciate and determine the prevalence and antibiotic resistant patterns of CoNS. Keywords:Coagulase-negative Staphylococci, Commensal, Speciation, antimicrobial susceptibility test
Authors and Affiliations
Dr. Satee shK
Keywords
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