Stimulate the antimicrobial activityof TiO2 nanoparticles (NPs) using methanol extract of Citrulluscolocynthis
Journal Title: Scholars Academic Journal of Pharmacy - Year 2016, Vol 5, Issue 8
Abstract
Abstract:Recently, great efforts have been made to develop nanomaterials with bioactive compounds to enhance the antimicrobial activity of the NPs to develop the microbial inactivation techniques.Four local bacterial strains gram positive and negative bacteria Staphylococcus aureus, Klebsiella pneumonia, Pseudomonousaeruginosaand E. coli were used to study the antibacterial activities of TiO2nanoparticles alone. The antimicrobial activity of Citrulluscolocynthis (methanol extract) alone and the combination treatment of on the viability of bacteria The morphologicalcharacteristicsof Tio2 NPs was determined via using Scanning electron microscope (SEM).Data showed that particle size is 50 nm in diameter with anatase morphology surface.Eighteen compounds were identified in crude alcohol fruit extract of C.colocynthis using GC-MS. The major component detected was 2H-1-Benzopyran-2-one, 7-methoxy- (51.57 %).Results indicate small inactivation effect were observed using TiO2(100mg/ml) NPs alone, the zone of inhibition (IZ) (19.3 mm), while a great inactivation effect was observed via using methanol extraction of C.colocynthis (100mg /ml) alone with (33 mm) IZ. The combination treatment of 50mg/ml of TiO2NPs and 50 mg/mlof methanol extraction of C.colocynthis showed antimicrobial effect against E. coli and S. aureuswith (39 mm) IZ using the well agar diffusion method. This concentration was located in synergic area of each two above bacteria. Klebsiella pneumonia and Pseudomonousaeruginosawere not sensitive to all treatments. Keywords:antimicrobial agents;Citrulluscolocynthis; combinations; GC/MS; TiO2 nanoparticles.
Authors and Affiliations
Dr. Raghad DH Abdul Jalill, Dr. Amna M Ali
Keywords
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