SUBSTRATE CARRIERS FOR C-1(2)-DEHYDROGENATION OF 6-METHYLENE ANDROSTENEDIONE TO EXEMESTANE BY GROWING AND IMMOBILIZED ARTHROBACTER SIMPLEX NCIM 2449
Journal Title: Asian Journal of Pharamceutical and Clinical Research - Year 2017, Vol 10, Issue 2
Abstract
Objective: Permeability of hydrophobic steroid substrates across cell membrane is a critical factor during microbial bioconversion. To increase substrate intake, the feasibility of some organic solvents and emulsifiers as substrate carrier on the bioconversion of 6-methylene androstenedione to exemestane was assessed.Methods: Androstenedione, a commonly available steroid precursor, was chemically converted 6-methylene androstenedione. The time course of exemestane accumulation was estimated after addition of 6-methylene androstenedione dissolved in some organic solvents or dispersed with emulsifiers by growing and immobilized cells of Arthrobacter simplex NCIM 2449 in shake flask cultures.  Results: The use of substrate carriers for addition of 6-methylene androstenedione enhanced the bioconversion several folds. With growing bacterium in triplicate flasks, a peak mol % bioconversion recorded was- ethanol (67.25, 72 h); soybean oil + tween 80 (50.37, 48 h); acetone (38.84, 48 h); soybean oil (38.36, 48 h); lecithin (32.73, 48 h), methanol (32.71, 48 h) and tween 80 (10.37, 48 h). As compared to the growing cells, the bioconversion with Ca-alginate immobilized cells was delayed and peak mol % bioconversion was recorded as ethanol (60.78, 120 h); soybean oil + tween 80 (42.98, 120 h);  methanol (40.50, 72 h);  soybean oil (38.36, 48 h);  acetone (31.18, 72h ) and lecithin (33.67, 120 h); tween 80 (13.87, 120 h).Conclusion: The use of substrate carriers for addition of 6-methylene androstenedione increased the permeability of substrate and may be used to increase the yield of exemestane and reduce incubation time.
Authors and Affiliations
Prachi Patil, Rajesh Sharma, Tushar Banerjee, Shridhar Patil
Keywords
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- EP ID EP605476
- DOI 10.22159/ajpcr.2017.v10i2.15861
- Views 90
- Downloads 0
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