Anti-Neuroinflammatory Effects of Sertoli Cells Improve Motor Activities and Striatal Volume in Rat Model of Huntington Disease
Journal Title: The 1st Annual Meeting of Georgian Center for Neuroscience Research - Year 2020, Vol 2, Issue 20
Abstract
Introduction: Huntington’s disease (HD) is a genetically heritable disorder, linked with continuing cell loss and degeneration mostly in the striatum. Currently, cell therapy approaches in HD have essentially been focused on replenishing or shielding cells lost over the period of the disease. Herein, we sought to explore the in vitro and in vivo efficacy of primary rat Sertoli cells (SCs) and their paracrine effect against oxidative stress with emphasis on HD induced by3-nitropropionic acid (3-NP). Methods: Initially, SCs were isolated and immunophenotypically characterized by positive expression of GATA4. Synthesis of neurotrophic factors of glial cell-derived neurotrophic factor and VEGF by SCs were confirmed. Then, PC12 cells were exposed to hydrogen peroxide in the presence of conditioned media (CM) collected from SC (SC-CM) and cell viability and neuritogenesis were determined. Finally, bilateral striatal implantation of SC in 3-NP-lesioned rat models was performed, and 1 month later, post-graft analysis was done. Results: Based on in vitro part of the current project, the CM of SC protected PC12 cells against oxidative stress, and it remarkably augmented cell viability and neurite outgrowth (fig.1). Furthermore, in vivo part of study showed grafted SCs survived, exhibited decreases in gliosis and inflammatory cytokine levels simultaneously (fig.2). Also, it ameliorated motor coordination and muscle activity in addition to an improvement in striatal volume in HD rats (fig.3). Conclusion: Our findings demonstrated that SCs might provide a supportive.
Authors and Affiliations
Amir-Hossein Bayat, Abbas Aliaghaei
Keywords
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- EP ID EP677936
- DOI 10.29088/GCNR-2020.27
- Views 201
- Downloads 2
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