Correlation between transaldolase and choroidal neovascularization and its mechanism in rat models
Journal Title: Eye Science (Yanke Xuebao) - Year 2020, Vol 35, Issue 6
Abstract
[Objective:] To observe the expression of transaldolase (TAL) in choroidal neovascularization (CNV) induced by krypton laser in BN rats and investigate its possible mechanism. [Methods:] Twenty rats in two groups were given with krypton laser photocoagulation retina to induce CNV BN rat models. After fluorescein fundus angiography (FFA) was performed 21 days after photocoagulation, the eyeballs were removed, and TAL, nicotinamide adenine dinucleotide phosphate (NADPH) and reduced glutathione (GSH) proteins were detected by Western blot, and TAL activity was determined. The siRNA was used to inhibit the expression of TAL in ARPE-19 cells. The changes of TAL, NADPH and GSH expression levels before and after inhibition were compared by Western blot. [Results:] Significant neovascularization was observed 21 days after photocoagulation. The TAL activity in the CNV group was significantly higher than that in the control group (67.16±3.38 vs 182.57±1.83, P<0.001); TAL protein expression in the CNV group was significantly higher than that in the control group (0.87±0.33 vs 2.09±0.21,P=0.005); NADPH and GSH expression in the CNV group was significantly lower than that in the control group (0.92±0.20 vs 0.14±0.05, P<0.01; 0.84±0.31 vs 0.29±0.11, P<0.05). After TAL-siRNA transfection of ARPE-19 cells for 48h, the expression of TAL protein in the CNV group was significantly lower than that of the control group and negative control group (0.26±0.13 vs 1.39±0.42 vs 1.15±0.19, P<0.01). The expression levels of NADPH and GSH in the CNV group were significantly higher than those in the control group (P<0.01) and negative control group (P<0.05). [Conclusion:] CNV is positively correlated with TAL expression, and TAL can participate in the development of CNV by regulating the expression levels of NADPH and GSH.
Authors and Affiliations
Ke QIN, Yan XU, Xiyuan ZHOU, Danning LIU
Keywords
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- EP ID EP763446
- DOI 10.3978/j.issn.1000-4432.2020.11.25
- Views 19
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