Follicular fluid cell free DNA in poly cystic ovary syndrome patients undergoing intracytoplasmic sperm injection program: relation to ovarian reserve and embryo quality
Journal Title: IOSR Journal of Pharmacy and Biological Sciences (IOSR-JPBS) - Year 2018, Vol 13, Issue 6
Abstract
Poly cystic ovary syndrome [PCOS] represents the most common disorder in women during reproductive age. Because of the expensiveness and invasiveness of in vitro fertilization procedures [IVF], it has been tried to find a trustful, non invasive way for choosing embryos. Cell free DNA [cfDNA] extracted from follicular fluid can provide an indicator for embryo quality. Ovarian reserve has an important role in the occurrence of pregnancy in IVF programs, the aim of the study is measure the amount of cfDNA in the follicular fluid in females with PCOS undergoing intracytoplasmic sperm injection program and relating it to the ovarian reserve and embryo quality. This prospective study was accomplished in AL-Samaray Teaching Hospital in Baghdad from December 2017 to March 2017. Sixty females were involved in this study. Thirty one did not have PCOS [control] and 29 had PCOS. Blood samples were withdrawn on day 3 of the cycle for hormonal analysis. All of the patients had entered through the ovarian stimulation protocol [antagonist protocol]. Follicular fluid was collected at time of oocyte retrieval; cfDNA was extracted, and calculated. Embryo quality was assessed too. In age matched POCS and control groups, the correlation between body mass index and cell free DNA in all cases was a non significant positive correlation [p˃0.05].There was also a non significant positive correlation between cfDNA and FSH level in both group [r=-0.080 and 0.228 respectively]. The correlation between AMH and cfDNA was a significant negative one in PCOS group while it was not significant in control group. there is a significant difference in cfDNA levels according to the embryo quality being less in grade1 and 2 embryos and more in bad grade 3 and 4 embryos in both groups.Cell free DNA in follicular fluid correlated negatively and significantly with the level of AMH in polycystic ovary syndrome patients, so the low levels of cfDNA indicates high ovarian reserve. The quality of embryos after intracytoplasmic sperm injection increased in embryos yielded from follicles with low levels of cfDNA.
Authors and Affiliations
Hanan L. Al- Omary, Shayma`a J. Ahmed, Maanee A. Al- azzam, Hiba R. Kassim
Keywords
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- DOI 10.9790/3008-1306020408.
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