Microstructural Tissue Assessment, Sex Hormones and Biochemical Investigations Following Acute Administration of Piper GuineenseSchumach&Thonn. on Female RattusNovergicus.
Journal Title: IOSR Journal of Applied Chemistry (IOSR-JAC) - Year 2018, Vol 11, Issue 5
Abstract
The effect of graded doses (50, 100 and 150mg/kg body weight) of crude ethanol extract of the dry seeds of Piper guineenseSchumach&Thonn was investigated on the ovary, liver, hormonal and some biochemical parameters of female rats weighing between 150-190g. Twenty rats were randomly divided into four groups of five rats each per group, group A (control) were orally dosed once daily with 1ml of distilled water (vehicle) while the test groups i.e. B, C and D were orally dosed with 50, 100 and 150mg/kg body weight of the crude extract in 1ml of the vehicle. After twenty one (21) days of the extract dosing samples were collected for analysis. The results are expressed as the mean of five replicates ± standard deviation, means were analyzed using one way analysis of variance (ANOVA) followed by Posthoc Turkey. p < 0.05 was regarded as significant. All doses of the extract caused significant increase𝑝 < 0.05 in a dose related manner on testosterone, luteinizing hormone, follicle stimulating hormone, progesterone and estradiol and at the same time reducing the prolactin level. AST, ALT and ALP were not significantly influenced 𝑝 > 0.05 except the 100 and 150mg/kg dose on ALP and only the 150mg/kg dose on the AST and ALT, all doses caused significant elevation (𝑝 < 0.05) in cholesterol and HDL while the 150mg/kg caused significant reduction in triglyceride and low density lipoprotein cholesterol, microstructural integrity assessment of the liver (H&E× 40) of the 150mg/kg group in comparison with the control group showed microvesicular steatosis (fatty change), in the same vein the ovary of this group reveals enlarged cells with poor follicle development.
Authors and Affiliations
Kpomah E. D
Keywords
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