Reaction mechanism of a protein error in the presence of in organic salt: proof from an absorption spectrum of color development
Journal Title: Journal of Analytical Bio-Science - Year 2012, Vol 35, Issue 5
Abstract
Color development by protein error decreases in the presence of in organic salt, and its reaction mechanism is proposed. In this paper, whether or not the absorption spectra of this color reaction indicates the characteristics estimated from the proposed reaction mechanism was investigated by experiments and calculations based on the chemical equilibrium. The change of absorption spectra in the presence of NaCl coincided with the characteristics estimated from the proposed reaction mechanism. This result shows that the coexisting anion competes with the dissociated dye anion in binding the positively charged protein.
Authors and Affiliations
Yuji Suzuki
Keywords
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