Spectrometric and RP-HPLC method for the determination of transresveratrol in vegetarian formulation
Journal Title: Indian Journal of Research in Pharmacy and Biotechnology - Year 2017, Vol 5, Issue 2
Abstract
Estimation of Trans-resveratrol was achieved by Spectrometric method. After considering the solubility studies, distilled water was selected as a solvent for the UV analysis. Trans-resveratrol 10μg/ml solution was prepared and scanned in the UV region from 180-380nm. From the spectrum, 307nm was selected as an analysing wavelength. Calibration curve was plotted by using concentration vs absorbance. From the calibration curve it was found that Trans-resveratrol obeys Beer’s and lambert’s law in the concentration range of 2-10μg/ml, molar absorptivitywas0.06938cm/mole, sandell’s sensitivity was 0.014442μg/cm2, correlation coefficient was 0.9977, LOD: 0.0665μg/ml, LOQ: 0.2016μg/ml were calculated. The amount of Transresveratrol in the vegetarian formulation was found to be 123.14mg/capsule. Trans-resveratrol was determinedby RP-HPLC method with external standard calibration method. Optimizations of chromatographic parameters were done. A wavelength of 305nm was selected as a detection wavelength. The mobile phase composition consisting of Acetonitrile: Phosphate buffer (pH-5) in the ratio of 28:72%v/v was selected for this proposed method.
Authors and Affiliations
Nallankala Sudha Rani, K. Narendra Kumar Reddy, Bada Pragati Kumar
Keywords
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